February 15, 2024
Identification of the native Torpedo californica nicotinic acetylcholine receptor’s glycan composition after a multi-step sequential purification method using MALDI-ToF MS. Proteomics

Maldonado-Hernández R, Quesada O, González-Feliciano JA, Baerga-Ortiz A, Lasalde-Dominicci JA.  2023 Oct 30:e2300151. doi: 10.1002/pmic.202300151. Epub ahead of print. PMID: 37904306.

February 7, 2024
Cholinergic Polarization of Human Macrophages

Roa-Vidal N, Rodríguez-Aponte AS, Lasalde-Dominicci JA, Capó-Vélez CM, Delgado-Vélez M. . Int J Mol Sci. 2023 Oct 29;24(21):15732. doi: 10.3390/ijms242115732. PMID: 37958716; PMCID: PMC10650439.

May 18, 2023
Assessment of Purity, Functionality, Stability, and Lipid Composition of Cyclofos-nAChR-Detergent Complexes from Torpedo californica Using Lipid Matrix and Macroscopic Electrophysiology

by Orestes Quesada · Joel E. González‑Nieves· José Colón · Rafael Maldonado‑Hernández · Carol González‑Freire · Jesús Acevedo‑Cintrón · Irvin D. Rosado‑Millán · José A. Lasalde‑Dominicci

May 18, 2023
Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles

by Pearl Akamine, José A. González-Feliciano, Ruth Almodóvar, Gloriner Morell, Javier Rivera, Coral M. Capó-Vélez, Manuel Delgado-Vélez, Luis Prieto-Costas, Bismark Madera, Daniel Eichinger, Ignacio Pino, José H. Rivera, José Ortiz-Ubarri, José M. Rivera, Abel Baerga-Ortiz and José A. Lasalde-Dominicci.

November 24, 2021
Pursuing High-Resolution Structures of Nicotinic Acetylcholine Receptors: Lessons Learned from Five Decades

Delgado-Vélez, M.; Quesada, O.; Villalobos-Santos, J.C.; Maldonado-Hernández, R.; Asmar-Rovira, G.; Stevens, R.C.; Lasalde Dominicci, J.A. Pursuing High-Resolution Structures of Nicotinic Acetylcholine Receptors: Lessons Learned from Five Decades. Molecules 2021, 26, 5753. https://doi.org/10.3390/molecules26195753

March 31, 2021
Disruption of the cholinergic anti-inflammatory response by R5-tropic HIV-1 protein gp120JRFL

Ríos SC, Colón Sáez JO, Quesada O, Figueroa KQ, Lasalde Dominicci JA. J Biol Chem. 2021 Jan-Jun;296:100618. doi: 10.1016/j.jbc.2021.100618. Epub 2021 Mar 31. PMID: 33811859 Free PMC article.

March 15, 2021
A reversed phase HPLC method for the quantification of HIV gp145 glycoprotein levels from cell culture supernatants

González-Feliciano JA, Capó-Vélez CM, Akamine P, Delgado-Vélez M, Almodóvar R, Rivera J, Pino I, Morell G, Eichinger D, Rivera JH, Lasalde-Dominicci JA, Baerga-Ortiz A. J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Mar 15;1167:122562. doi: 10.1016/j.jchromb.2021.122562. Epub 2021 Jan 27. PMID: 33571843.

December 1, 2020
Sequential purification and characterization of Torpedo californica nAChR-DC supplemented with CHS for high-resolution crystallization studies

Maldonado-Hernández R, Quesada O, Colón-Sáez JO, Lasalde-Dominicci JA. Anal Biochem. 2020 Dec 1;610:113887. doi: 10.1016/j.ab.2020.113887. Epub 2020 Aug 4. PMID: 32763308.

August 27, 2020
Biophysical characterization dataset of native nicotinic acetylcholine receptor in lipid-like detergent complexes

Maldonado-Hernández R, Quesada O, Lasalde-Dominicci JA. Data Brief. 2020 Aug 27;32:106230. doi: 10.1016/j.dib.2020.106230. eCollection 2020 Oct. PMID: 32939382.

June 19, 2020
A recombinant gp145 Env glycoprotein from HIV-1 expressed in two different cell lines: Effects on glycosylation and antigenicity

González-Feliciano JA, Akamine P, Capó-Vélez CM, Delgado-Vélez M, Dussupt V, Krebs SJ, Wojna V, Polonis VR, Baerga-Ortiz A, Lasalde-Dominicci JA. PLoS One. 2020 Jun 19;15(6):e0231679. doi: 10.1371/journal.pone.0231679. eCollection 2020. PMID: 32559193.

October 22, 2018
Amphetamine enantiomers inhibit homomeric α7 nicotinic receptor through a competitive mechanism and within the intoxication levels in humans.

Garton DR, Ross SG, Maldonado-Hernández R, Quick M, Lasalde-Dominicci JA, Lizardi-Ortiz JE.Neuropharmacology. 2018 Oct 22. pii: S0028-3908(18)30819-0. doi: 10.1016/j.neuropharm.2018.10.032. [Epub ahead of print]PMID:30359640

July 14, 2018
Nicotinic Acetylcholine Receptors in HIV: Possible Roles During HAND and Inflammation.

Capó-Vélez CM, Delgado-Vélez M, Báez-Pagán CA, Lasalde-Dominicci JA. Cell Mol Neurobiol. 2018 Jul 14. doi: 10.1007/s10571-018-0603-8.

May 16, 2018
The Cholinergic Anti-Inflammatory Response and the Role of Macrophages in HIV-Induced Inflammation

Manuel Delgado-Vélez  and José A. Lasalde-Dominicci. Sci Rep. 2018 Jan 29:8(1):1829. Int J Mol Sci. 2018 May 16: 19(5)

January 29, 2018
The alpha7-nicotinic receptor contributes to gp120-induced neurotoxicity: implications in HIV-associated neurocognitive disorders

Coral M. Capó-Vélez, Bryan Morales-Vargas, Aurian García-González, José G. Grajales-Reyes, Manuel Delgado-Vélez, Bismark Madera, Carlos A. Báez-Pagán, Orestes Quesada & José A. Lasalde-Dominicci

November 21, 2017
A Panel of Slow-Channel Syndrome Mice Reveals a Unique Locomotor Behavioral Signature.

Grajales-Reyes JG, García-González A, María-Ríos JC, Grajales-Reyes GE, Delgado-Vélez M, Báez-Pagán CA, Quesada O, Gómez CM, Lasalde-Dominicci JA. J Neuromuscul Dis. 2017;4(4):341-347.

September 21, 2016
Inflamación periferal inducida por VIH: un problema sin resolver.

Delgado-Vélez, M and Lasalde-Dominicci, JA. Revista Puertorriqueña de Medicina y Salud Pública. (2016). Mar. Vol 54, 92-96.

April 26, 2016
Heterogeneous Inhibition in Macroscopic Current Responses of Four Nicotinic Acetylcholine Receptor Subtypes by Cholesterol Enrichment

Báez-Pagán, C.A., Del Hoyo-Rivera, N., Quesada, O., Lasalde-Dominicci, J.A.
J Membr Biol. 2016 Aug;249(4):539-49.

December 25, 2015
Functionality and stability data of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology.

Luis F. Padilla-Morales, José O. Colón-Sáez, Joel E. González-Nieves, Orestes Quesada-González, José A. Lasalde-Dominicci Data in Brief 6 (2016) 433-437

December 11, 2015
The α7-nicotinic receptor is upregulated in immune cells from HIV-seropositive women: consequences to the cholinergic anti-inflammatory response.

Delgado-Vélez M, Báez-Pagán CA, Gerena Y, Quesada O, Santiago-Pérez LI, Capó-Vélez CM, Wojna V, Meléndez L, León-Rivera R, Silva W, Lasalde-Dominicci JA. Clin Transl Immunology. 2015 Dec 11;4(12):e53.

November 13, 2015
Decreased glial and synaptic glutamate uptake in the striatum of HIV-1 gp120 transgenic mice.

Melendez RI, Roman C, Capó-Vélez CM, Lasalde-Dominicci JA.
J Neurovirol. 2016. Jun; 22(3):35865

November 13, 2015
Expression of CHRFAM7A and CHRNA7 in neuronal cells and postmortem brain of HIV-infected patients: considerations for HIV-associated neurocognitive disorder.

Ramos FM, Delgado-Vélez M, Ortiz ÁL, Báez-Pagán CA, Quesada O, Lasalde-Dominicci JA. J Neurovirol. 2015 Nov 13. PMID:26567012

October 8, 2015
Assessment of the functionality and stability of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology.

Padilla-Morales LF, Colón-Sáez JO, González-Nieves JE, Quesada-González O, Lasalde-Dominicci JA. Biochim Biophys Acta. 2016 Jan;1858(1):47-56.

September 19, 2015
Uncovering the lipidic basis for the preparation of functional nicotinic acetylcholine receptor detergent complexes for structural studies.

Quesada O, González-Freire C, Ferrer MC, Colón-Sáez JO, Fernández-García E, Mercado J, Dávila A, Morales R, Lasalde-Dominicci JA.Sci Rep. 2016 Sep 19;6:32766.

September 9, 2015
Lateral Diffusion, Function, and Expression of the Slow Channel Congenital Myasthenia Syndrome αC418W Nicotinic Receptor Mutation with Changes in Lipid Raft Components.

Oyola-Cintrón J, Caballero-Rivera D, Ballester L, Baéz-Pagán CA, Martínez HL, Vélez-Arroyo KP, Quesada O, Lasalde-Dominicci JA. J Biol Chem. 2015 Oct 30;290(44):26790-800.

April 24, 2015
Engineering α4β2 nAChRs with reduced or increased nicotine sensitivity via selective disruption of consensus sites in the M3-M4 cytoplasmic loop of the α4 subunit.

Biaggi-Labiosa NM, Avilés-Pagán E, Caballero-Rivera D, Báez-Pagán CA, Lasalde-Dominicci JA. Neuropharmacology. 2015 Dec;99:273-84.

April 14, 2015
Activation of the Macrophage α7 Nicotinic Acetylcholine Receptor and Control of Inflammation.

Báez-Pagán CA, Delgado-Vélez M, Lasalde-Dominicci JA.
J Neuroimmune Pharmacol. 2015 Sep;10(3):468-76.

October 23, 2014
Fluoxetine is neuroprotective in slow-channel congenital myasthenic syndrome

Zhu H, Grajales-Reyes GE, Alicea-Vázquez V, Grajales-Reyes JG, Robinson K, Pytel P, Báez-Pagán CA, Lasalde-Dominicci JA, Gomez CM. Exp Neurol. 2015 Aug;270:88-94.

April 13, 2012
Transgenic mouse model reveals an unsuspected role of the acetylcholine receptor in statin-induced neuromuscular adverse drug reactions. The Pharmacogenomics Journal

GE Grajales-Reyes, CA Baez-Pagan, H Zhu, JG Grajales-Reyes, M Delgado-Velez, WF Garcıa-Beltran, CA Luciano,
O Quesada, R Ramırez, CM Gomez and JA Lasalde-Dominicci Aug;13(4):362-8

March 6, 2012
Tryptophan scanning mutagenesis reveals distortions in the helical structure of the δM4 transmembrane domain of the Torpedo californica nicotinic acetylcholine receptor

Caballero-Rivera, D., Cruz-Nieves, O.A., Oyola-Cintrón, J., Torres-Nunez, D.A., Otero-Cruz, J.D., Lasalde-Dominicci, J.A. (2012) Channels (Austin) 2012 Mar-Apr 6(2):111-23

November 30, 2011
SorLA in glia: shared subcellular distribution patterns with caveolin-1.

Salgado I.K., Serrano M., Garcia J.O., Martinez N.A., Maldonado H.M., Báez-Pagán C.A., Lasalde-Dominicci J.A. (2011) SorLA in Glia.

September 16, 2011
Effects of lipid-analog detergent solubilization on the functionality and lipidic cubic phase mobility of the Torpedo californica nicotinic acetylcholine receptor.

Padilla-Morales L.F., Morales-Pérez C.L., De La Cruz-Rivera P.C., Asmar-Rovira G., Báez-Pagán C.A., Quesada O., and Lasalde-Dominicci J.A. (2011)

July 1, 2011
Fourier transform coupled tryptophan scanning mutagenesis identifies a bending point on the lipid-exposed δM3 transmembrane domain of the Torpedo californica nicotinic acetylcholine receptor.

Caballero-Rivera D., Cruz-Nieves O.A., Oyola-Cintrón J., Torres-Núñez D.A., Otero-Cruz J.D., and Lasalde-Dominicci J.A. (2011) Channels (Austin). Jul-Aug;5(4):345-56.

January 1, 2011
Up-regulation of the Neuronal Nicotinic Receptor α7 by HIV Glycoprotein 120: potential implications for HIV-associated neurocognitive disorder.

Ballester L.Y., Capo-Velez C.M., Garcia-Beltran W.F., Ramos F.M., Vazquez-Rosa E., Rios R., Mercado J.R., Melendez R.I. and Lasalde-Dominicci J.A. Jan 27;287(5):3079-86

January 17, 2010
Decoding pathogenesis of slow-channel congenital myasthenic syndromes using recombinant expression and mice models.

Otero-Cruz J.D., Baez-Pagan C.A., Dorna-Perez L., Grajales-Reyes G.E., Ramirez-Ordoñez R.T., Luciano C.A., Gomez C.M., and Lasalde-Dominicci J.A. (2010)  P R Health Sci J. (1):4-17. Review.

November 26, 2009
Triiodothyronine (T3) action on aquatic locomotor behavior during metamorphosis of the bullfrog Rana catesbeiana

Fernandez-Mongil, M., Venza, C. J., Rivera, A., Lasalde-Dominicci, J. A., Burggren, W., and Rojas, L. V., (2009) 53(1):101-8.

July 8, 2009
Contribution of Position α4S336 on Functional Expression and Up-regulation of α4β2 Neuronal Nicotinic Receptors

Lopez-Hernandez G.Y., Biaggi-Labiosa N.M., Torres-Cintron A., Ortiz-Acevedo A., and Lasalde-Dominicci J.A. (2009)

July 26, 2008
Aromaticity at the water-hydrocarbon core interface of the membrane: Consequences on the nicotinic acetylcholine receptor

Lizardi-Ortiz, J. E., Hyzinski-Garcia, M. C., Fernandez-Gerena, J. L., Osorio-Martinez, K. M., Velazquez-Rivera, E., Valle-Aviles, F. L., and Lasalde-Dominicci, J. A. (2008)  Channels (Austin) 2, 191-201.

July 18, 2008
Contribution of Position α4S336 on Functional Expression and Up-regulation of α4β2 Neuronal Nicotinic Receptors

Lopez-Hernandez, G. Y., Biaggi-Labiosa, N. M., Torres-Cintron, A., Ortiz-Acevedo, A., and Lasalde-Dominicci, J. A. (2008) Cell Mol Neurobiol.

May 5, 2008
Fourier transform coupled to tryptophan-scanning mutagenesis: lessons from its application to the prediction of secondary structure in the acetylcholine receptor lipid-exposed transmembrane domains

Otero-Cruz, J. D., Torres-Nunez, D. A., Baez-Pagan, C. A., and Lasalde-Dominicci, J. A. (2008)  Biochim Biophys Acta 1784, 1200-1207.

May 3, 2008
Potential role of caveolin-1-positive domains in the regulation of the acetylcholine receptor’s activatable pool: Implications in the pathogenesis of a novel congenital myasthenic syndrome

Baez-Pagan, C. A., Martinez-Ortiz, Y., Otero-Cruz, J. D., Salgado-Villanueva, I. K., Velazquez, G., Ortiz-Acevedo, A., Quesada, O., Silva, W. I., and Lasalde-Dominicci, J. A. Channels (Austin) 2, 180-190.

April 9, 2008
Tryptophan scanning of the acetylcholine receptor’s βM4 transmembrane domain: Decoding allosteric linkage at the lipid-protein interface with ion-channel gating

Diaz-De Leon, R., Otero-Cruz, J. D., Torres-Nunez, D. A., Casiano, A., and Lasalde-Dominicci, J. A. (2008) Channels (Austin) 2.

April 7, 2008
Functional contribution of α3L8′ to the neuronal nicotinic α3 receptor

Nieves-Cintron, M., Caballero-Rivera, D., Silva, W. I., Navedo, M. F., and Lasalde-Dominicci, J. A. (2008) J Neurosci Res 86, 2884-2894.

March 25, 2007
Cognitive deficits in schizophrenia: focus on neuronal nicotinic acetylcholine receptors and smoking

Ochoa, E. L., and Lasalde-Dominicci, J. 2007 Aug; 27(5): 609-39 Review

March 20, 2007
Tryptophan-scanning mutagenesis in the αM3 transmembrane domain of the muscle-type acetylcholine receptor. A spring model revealed

Otero-Cruz, J. D., Baez-Pagan, C. A., Caraballo-Gonzalez, I. M., and Lasalde-Dominicci, J. A.  9162-9171.

March 7, 2007
Associations of cigarette smoking with viral immune and cognitive function in human immunodeficiency virus-seropositive women

Wojna, V., Robles, L., Skolasky, R. L., Mayo, R., Selnes, O., de la Torre, T., Maldonado, E., Nath, A., Melendez, L. M., and Lasalde-Dominicci, J. J Neurovirol 13, 561-568.

July 2, 2006
The polarity of lipid-exposed residues contributes to the functional differences between Torpedo and muscle-type nicotinic receptors

Guzman, G. R., Ortiz-Acevedo, A., Ricardo, A., Rojas, L. V., and Lasalde-Dominicci, J. A.

May 20, 2006
Novel β subunit mutation causes a slow-channel syndrome by enhancing activation and decreasing the rate of agonist dissociation

Navedo, M. F., Lasalde-Dominicci, J. A., Baez-Pagan, C. A., Diaz-Perez, L., Rojas, L. V., Maselli, R. A., Staub, J., Schott, K., Zayas, R., and Gomez, C. M. 82-90.

May 16, 2006
Contribution of valine 7′ of TMD2 to gating of neuronal α3 receptor subtypes

Nieves-Cintron, M., Caballero-Rivera, D., Navedo, M. F., and Lasalde-Dominicci, J. A.

April 3, 2006
Macroscopic properties of spontaneous mutations in slow-channel syndrome: correlation by domain and disease severity

Zayas, R., Lasalde-Dominicci, J., and Gomez, C. M. (2006) 441-449.

August 11, 2004
Tryptophan scanning mutagenesis in the TM3 domain of the Torpedo californica acetylcholine receptor beta subunit reveals an α-helical structure

Santiago, J., Guzman, G. R., Torruellas, K., Rojas, L. V., and Lasalde-Dominicci, J. A.

July 9, 2004
Nicotine-induced Up-regulation and Desensitization of α4β2 Neuronal Nicotinic Receptors Depend on Subunit Ratio*

Gretchen Y. López-Hernández, Javier Sánchez-Padilla, Alejandro Ortiz-Acevedo, José Lizardi-Ortiz, Janice Salas-Vincenty, Legier V. Rojas and José A. Lasalde-Dominicci

March 18, 2004
Tryptophan scanning mutagenesis in the TM3 domain of the Torpedo californica acetylcholine receptor beta subunit reveals an α-helical structure.

Navedo, M., Nieves, M., Rojas, L., and Lasalde-Dominicci, J. A.

February 26, 2004
Tryptophan scanning mutagenesis of the γM4 transmembrane domain of the acetylcholine receptor from Torpedo californica

Ortiz-Acevedo, A., Melendez, M., Asseo, A. M., Biaggi, N., Rojas, L. V., and Lasalde-Dominicci, J. A.

September 7, 2003
Tryptophan scanning mutagenesis in the αM3 transmembrane domain of the Torpedo californica acetylcholine receptor: functional and structural implications

Guzman, G. R., Santiago, J., Ricardo, A., Marti-Arbona, R., Rojas, L. V., and Lasalde-Dominicci, J. A.

August 3, 2003
Thyroid hormones regulate the frequency of miniature end-plate currents in pre- and prometamorphic stages of the tadpole tail

Rojas, L. V., Bonilla, L., Baez, S., and Lasalde-Dominicci, J. A.

December 5, 2002
Regulation of Acetylcholine Receptor function by Cholesterol. In: Recent Research Developments in Membrane Biology: ISBN: 81-7736-181-3, p127-146

Guzmán, G. M., Ortiz-Acevedo, A., Santiago, J., Rojas, L.V. and Lasalde-Dominicci, J. A.

November 17, 2002
Novel delta subunit mutation in slow-channel syndrome causes severe weakness by novel mechanisms

Gomez, C. M., Maselli, R. A., Vohra, B. P., Navedo, M., Stiles, J. R., Charnet, P., Schott, K., Rojas, L., Keesey, J., Verity, A., Wollmann, R. W., and Lasalde-Dominicci, J.

June 24, 2001
Tryptophan substitutions at lipid-exposed positions of the gamma M3 transmembrane domain increase the macroscopic ionic current response of the Torpedo californica nicotinic acetylcholine receptor

Cruz-Martin, A., Mercado, J. L., Rojas, L. V., McNamee, M. G., and Lasalde-Dominicci, J. A.

February 15, 2001
Probing the effects of membrane cholesterol in the Torpedo californica acetylcholine receptor and the novel lipid-exposed mutation α C418W in Xenopus oocytes

Santiago, J., Guzman, G. R., Rojas, L. V., Marti, R., Asmar-Rovira, G. A., Santana, L. F., McNamee, M., and Lasalde-Dominicci, J. A.

July 8, 2000
Functional effects of periodic tryptophan substitutions in the α M4 transmembrane domain of the Torpedo californica nicotinic acetylcholine receptor

Tamamizu, S., Guzman, G. R., Santiago, J., Rojas, L. V., McNamee, M. G., and Lasalde-Dominicci, J. A.

March 3, 1999
Alteration in ion channel function of mouse nicotinic acetylcholine receptor by mutations in the M4 transmembrane domain

Tamamizu, S., Lee, Y., Hung, B., McNamee, M. G., and Lasalde-Dominicci, J. A.

August 3, 1997
Mutations in the M4 domain of the Torpedo californica nicotinic acetylcholine receptor alter channel opening and closing

Ortiz-Miranda, S. I., Lasalde, J. A., Pappone, P. A., and McNamee, M. G.

June 25, 1997
Mouse-Torpedo chimeric α-subunit used to probe channel-gating determinants on the nicotinic acetylcholine receptor primary sequence

Butler, D. H., Lasalde, J. A., Butler, J. K., Tamamizu, S., Zimmerman, G., and McNamee, M. G.

April 8, 1997
Slow-channel transgenic mice: a model of postsynaptic organellar degeneration at the neuromuscular junction

Gomez, C. M., Maselli, R., Gundeck, J. E., Chao, M., Day, J. W., Tamamizu, S., Lasalde, J. A., McNamee, M., and Wollmann, R. L.  PMID: 9151734.

December 18, 1996
A beta-subunit mutation in the acetylcholine receptor channel gate causes severe slow-channel syndrome, Ann Neurol 39, 712-723.

Gomez, C. M., Maselli, R., Gammack, J., Lasalde, J., Tamamizu, S., Cornblath, D. R., Lehar, M., McNamee, M., and Kuncl, R. W.

December 4, 1996
Tryptophan substitutions at the lipid-exposed transmembrane segment M4 of Torpedo californica acetylcholine receptor govern channel gating

Lasalde, J. A., Tamamizu, S., Butler, D. H., Vibat, C. R., Hung, B., and McNamee, M. G.

October 2, 1996
Effects of antibody binding on structural transitions of the nicotinic acetylcholine receptor

Tamamizu, S., Butler, D. H., Lasalde, J. A., and McNamee, M. G.

November 12, 1995
Heterogeneous distribution of acetylcholine receptors in chick myocytes induced by cholesterol enrichment

Lasalde, J. A., Colom, A., Resto, E., and Zuazaga, C.

September 2, 1995
Differential desensitization properties of rat neuronal nicotinic acetylcholine receptor subunit combinations expressed in Xenopus laevis oocytes

Vibat, C. R., Lasalde, J. A., McNamee, M. G., and Ochoa, E. L.

January 12, 1994
Mutations in the M4 domain of Torpedo californica acetylcholine receptor dramatically alter ion channel function

Lee, Y. H., Li, L., Lasalde, J., Rojas, L., McNamee, M., Ortiz-Miranda, S. I., and Pappone, P.