A recent article “Uncovering the lipidic basis for the preparation of functional nicotinic acetylcholine receptor detergent complexes for structural studies” has been accepted in Scientific Reports, an open access journal from the publishers of Nature.
Orestes Quesada 1* , Carol González 2 , María Carla Ferrer 3 , José Colón 3 , Emily Fernández Garcia 3 , Juan Mercado 3 , Alejandro Dávila 3 , Reginald Morales 2 and José A. Lasalde-Dominicci 3*
1 Department of Physical Sciences, University of Puerto Rico, Río Piedras Campus, PO Box 23323, San Juan, Puerto Rico, 00931-03323; 2 Department of Chemistry, University of Puerto Rico, Río Piedrasn Campus, PO Box 23346, San Juan, Puerto Rico, 00931-3346; 3 Department of Biology, University of Puerto Rico, Río Piedras Campus, PO Box 70377, San Juan, Puerto Rico, 00936-8377
This study compares the lipid composition, including individual phospholipid molecular species of solubilized nAChR detergent complexes (nAChR-DCs) with those of the bulk lipids from their source, Torpedo californica (Tc) electric tissue. This lipidomic analysis revealed seventy-seven (77) phospholipid species in the Tc tissue. Analysis of affinity purified nAChR-DCs prepared with C-12 to C-16 phospholipid analog detergents alkylphosphocholine (FC) and lysofoscholine (LFC) demonstrated that nAChR-DCs prepared with FC12, LFC14 and LFC16 contained >60 phospholipids/nAChR, which was more than twice of those prepared with FC14, FC16, and LFC12. Significantly, all the nAChR-DCs lacked ethanolamine and anionic phospholipids, contained only four cholesterol molecules, and a limited number of phospholipid molecular species per nAChR. Upon incorporation into oocytes, only FC12 is similar to the crude, whereas LFC14 and LFC16 nAChR-DCs displayed an increased functionality as compared to the crude Tc membrane. All three nAChR-DC displayed different degrees of alterations in macroscopic activation and desensitization kinetics.